Biological evaluation of artificial skin substitute / 生物医学工程学杂志

Multiple kinds of Artificial Skin Substitute are now available. However, except for the Homo Skin Graft there is no Artificial Skin Substitute that can be used as permanent Artificial Skin Substitute. During the past 20 years, more and more scholars around the world have expressed increased interests in the research and development of Artificial Skin Graft that can be utilized as satisfying permanent Artificial Skin Substitute. We conducted our research on the biological evaluation of medical devices of Collagen-Chitosan(C-C) Artificial Skin Substitute according to the National Standard (GB/T16886. 1-1997). The following experiments were conducted: (1)Cytotoxicity, (2)Systemic toxicity(acute toxicity), (3)Haemocompatibility, (4)Sensitization, (5)Intracutaneous reactivity, (6)Pyrogen test, (7)Genotoxicity. The experiment results demonstrate that all biological functional indexes of the Artificial Skin Graft meet the National Standards. Therefore, we conclude that C-C Artificial Skin Graft is characteristic of good biological compatibility. It is non-irritant and has no systemic and cellular toxicity, no genotoxicity, no pyrogen, and no allergen.

The neuroprotective effects and its mechanisms of qingkailing injection on bacterial meningitis induced by E. coli in rabbits / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the neuro-protective effect and mechanism of qingkailing injection (QKL) against cerebral injury caused by E. coli-meningitis (CM).</p><p><b>METHODS</b>The CM model rabbits were treated by ampicillin with QKL as adjuvant. The leukocyte count and protein content in cerebral spinal fluid (CSF), the contents of water, sodium, potassium and calcium in cerebral tissues were measured before, 16 h and 26 h after Bacillus coli injection respectively. The expression of matrix metalloproteinase-9 (MMP-9) was determined at the same time.</p><p><b>RESULTS</b>Adjunctive treatment with QKL can not only inhibit the increase of leukocyte cells, protein content in CSF, and water, sodium, calcium content in cerebral tissues, but also the decrease of potassium content revealed during simple antibiotic treatment. It also can decrease the expression of MMP-9 in cerebral tissues of rabbits with CM.</p><p><b>CONCLUSION</b>As an adjunctive treatment, QKL can prevent transient inflammatory reaction and aggravation of brain injury in CM induced by simple antibiotic treatment, its mechanisms might relate with calcium antagonism and attenuation of MMP-9 expression in brain tissues.</p>

Analysis of the efficacy of lung-protective ventilation strategy in burn patients with respiratory dysfunction / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the efficacy of lung-protective ventilation strategy in burn patients with respiratory dysfunction.</p><p><b>METHODS</b>Mechanical ventilation was employed in 11 cases of severely burned patients with the pattern of synchronized intermittent mandatory ventilation and pressure support ventilation (SIMV + PSV). The tidal volume was set at 6 approximately 8 ml/kg and inspiratory plateau pressure was equal to or less than (</=) 30 cmH(2)O. PEEP was set at 8 approximately 10 cmH(2)O and FiO(2) 40% approximately 50%. Blood gas analysis indices were dynamically monitored and clinical outcome was observed.</p><p><b>RESULTS</b>SIMV and PSV were applied in these patients for an average of 12.77 +/- 8.06 days. After treatment, the PaO(2)/FiO(2) ratio was obviously increased from 193.54 +/- 53.3 to 327.14 +/- 106.53 (P < 0.01). Furthermore, the PaCO(2) and pH values remained at normal range. Five out of 11 patients finally died, in which 3 were due to renal failure and one due to multiple organ dysfunction (MOD).</p><p><b>CONCLUSION</b>Respiratory support in severely burned patients could be achieved by lung-protective ventilation strategy.</p>

A multicenter clinical trial of piperacillin/tazobactam in burn infection / 中华烧伤杂志

<p><b>OBJECTIVE</b>To evaluate the efficacy of piperacillin/tazobactam in the management of burn infection.</p><p><b>METHODS</b>Sixty-three burn patients were enrolled in the study with burn sepsis or burn area more than 50%TBSA or full skin loss more than 30% TBSA. The administration regime of the antibiotics was 4.5 g intravenously administered every 8 hours in the treatment of burn sepsis or in the prophylactic management. The effectiveness was identified when the septic symptoms disappeared or focal infection did not develop into sepsis.</p><p><b>RESULTS</b>The overall clinical efficacy was 90.4%, and success in sepsis (control) was 75%. Furthermore, 95.7% of the focal infection was prevented from developing into systemic infection. The bacterial clearance rates were 71.4% and 51.4% in treatment and prophylaxis groups, respectively.</p><p><b>CONCLUSION</b>Piperacillin/taxobactam was effective in the treatment and/or prophylaxis of burn sepsis caused by bacteria susceptible to it, so it could be applied empirically.</p>